ATTENUATION OF MEDIAL FOREBRAIN-BUNDLE REWARD BY ANTERIOR LATERAL HYPOTHALAMIC-LESIONS

Psychophysical data consistent with rostro-caudal conduction along rew ard-relevant neurons linking the lateral hypothalamus (LH) and ventral tegmental area (VTA) have lead to the hypothesis that some of the dir ectly activated neurons responsible for medial forebrain bundle (MFB) self-stimulation arise anterior io the level of the LH. This hypothesi s has been challenged on the grounds that lesions to the anterior LH ( ALH) often fail to degrade the rewarding value of stimulating more pos terior MFB sites. The present study was aimed at investigating the eff ect of lesion location and stimulation current on the efficacy of ALH lesions in an effort to account for the inconsistencies in the earlier data. Self-stimulation thresholds were obtained for LH and VTA sites by estimating the number of pulses per stimulation train required for half-maximal responding at each of 3 currents. Electrolytic lesions (a nodal, 1.0 mA for 10 s) were then made to the ALH at varying medial-la teral coordinates. In 7 of the 14 rats with MFB stimulation sites, les ions to the ALH produced increases in threshold which often declined o ver the next several days to weeks; in 5 cases thresholds remained ele vated by 0.1 to 0.25 log(10) units above baseline up to the end of tes ting. In ail but one case, the effective lesions were centered in the lateral ALH. Increases in threshold were more likely to be detected wh en stimulating at low currents; at low currents fewer neurons are recr uited and the lesion can have a greater proportional effect on thresho ld. These data support the hypothesis that cell bodies, terminals, or fibers of passage in the ALH contribute to the rewarding effect of sti mulating more posterior MFB sites.