Ja. Guisantes et al., APPLICATION OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ASSAY TO THE STUDY OF MITES RELATED TO ALLERGIC DISEASES, Journal of investigational allergology & clinical immunology, 6(1), 1996, pp. 65-68
In this study, the conditions for the successful application of the ra
ndom amplified polymorphic DNA (RAPD) assay to differentiate mite popu
lations based on genetic variation were defined. Five species of mites
related to allergic diseases were studied: Dermatophagoides pteronyss
inus, D. farinae (2 strains), Blomia tropicalis, Glycyphagus domesticu
s and Tyrophagus putrescentiae. The mites were isolated from pure cult
ures and processed according to the method described in this paper. Th
e banding patterns obtained were different for all the species studied
. When the DNA from two different strains of D. farinae were studied,
the ''fingerprint'' banding patterns obtained showed differences betwe
en them. The random amplified polymorphic DNA assay may be a useful to
ol to aid the taxonomic study of mite populations.