ADHERENCE OF HUMAN PROSTASOMES TO MOUSE SPERMATOZOA AND THEIR DISPLACEMENT BY MONOCLONAL-ANTIBODIES AS REVEALED BY FREE ZONE ELECTROPHORESIS

Prostasomes, small corpuscular organelles derived from the prostate gl and, were isolated from human seminal plasma by means of ultracentrifu gation and Sephadex G 200 chromatography to examine their interactive ability with mouse spermatozoa as assessed by free zone electrophoresi s. During the run, human prostasomes and mouse spermatozoa approached each other and finally fused into one single peak that was not further dissociated. In some cases the fusion of prostasomes and spermatozoa failed to appear fell owing pretreatment of prostasomes with monoclona l antibodies raised against these organelles.