ASSESSMENT OF ANEUPLOIDY FOR CHROMOSOME-8, CHROMOSOME-9, CHROMOSOME-13, CHROMOSOME-16, AND CHROMOSOME-21 IN HUMAN SPERM BY USING PRIMED IN-SITU LABELING TECHNIQUE

The incidence of aneuploidy was estimated for chromosomes 8, 9, 13, 16 , and 21 in mature human spermatozoa by primed in situ (PRINS) labelin g technique. This method allows us to perform a chromosome-specific de tection by in situ annealing of a centromeric specific primer. A dual color PRINS protocol was adapted to human sperm. The decondensation an d the denaturation of sperm nuclei were simultaneously performed by 3- M NaOH treatment. Double labeling of spermatozoa was obtained in <2 h. A total of 96,292 sperm nuclei were analyzed by two independent obser vers. The estimates of disomy were 0.31% for chromosome 8, 0.28% for c hromosome 9, 0.28% for chromosome 13, 0.26% for chromosome 16, and 0.3 2% for chromosome 21. These homogeneous findings suggest an equal dist ribution of aneuploidies among all autosomal chromosomes in males.