Pm. Mele et Mr. Carter, ESTIMATION OF MICROBIAL BIOMASS BY NINHYDRIN-REACTIVE N USING LIQUID CHLOROFORM, Canadian Journal of Soil Science, 76(1), 1996, pp. 37-40
Ninhydrin-reactive N (NRN), which is a reliable and sensitive indicato
r of soil microbial biomass, was measured in extracts of three Austral
ian duplex soils, under different tillage systems, fumigated using eit
her vaporized or liquid CHCl3. Although the relationship between the t
wo fumigation-extraction methods was influenced by soil type, the use
of liquid CHCl3 appears to be a promising technique to allow greater e
ase and speed for releasing NRN in microbial biomass analysis.