AN ELECTROGENERATED CHEMILUMINESCENCE PROBE AND ITS APPLICATION UTILIZING TRIS(2,2'-BIPYRIDYL)RUTHENIUM(II) AND LUMINOL CHEMILUMINESCENCE WITHOUT A FLOWING STREAM

A novel electrogenerated chemiluminescent (ECL) probe that does not re quire a dark box to exclude ambient light has been developed. The prob e is placed within the sample solution in a fashion similar to the use of a pH electrode. Thus, the flowing stream method of analyte introdu ction typically used for ECL analysis is not necessary. A fiber-optic bundle is utilized to carry the light produced within the probe to a p hotomultiplier tube for detection. Within the body of this probe is a three-electrode configuration necessary for ECL generation. The three ECL systems, luminol, tris(2,2'-bipyridyl)ruthenium(III), and luminol coupled with an immobilized oxidase enzyme, have been used with this p robe. The relative standard deviation for a typical sample was found t o be 0.6%, Glucose determinations ranging from 3.0 to 1000 mu M were m ade without a pH change for several real samples employing luminol and glucose oxidase immobilized within the probe body.