ENZYMATIC AMPLIFICATION AND MOLECULAR-CLONING OF CDNA-ENCODING THE SMALL AND LARGE SUBUNITS OF BOVINE INTERLEUKIN-12

Citation
Ds. Zarlenga et al., ENZYMATIC AMPLIFICATION AND MOLECULAR-CLONING OF CDNA-ENCODING THE SMALL AND LARGE SUBUNITS OF BOVINE INTERLEUKIN-12, Biochimica et biophysica acta. Molecular basis of disease, 1270(2-3), 1995, pp. 215-217
Citations number
8
ISSN journal
09254439
Volume
1270
Issue
2-3
Year of publication
1995
Pages
215 - 217
Database
ISI
SICI code
0925-4439(1995)1270:2-3<215:EAAMOC>2.0.ZU;2-D
Abstract
cDNA generated from stimulated abomasal lymph node cells was used to a mplify and clone the 35 kDa and 40 kDa subunits of bovine interleukin 12 (IL-12) using primers derived from semi-conserved regions between h uman and mouse IL-12 sequences. The deduced amino acid sequence of the 40 kDa subunit demonstrated 84.4% and 67.6% homology with human and m ouse sequences, respectively. The deduced sequence of the 35 kDa subun it exhibited comparable similarities to the human 35 kDa subunit (82.2 %) but differed significantly (58.6%) from mouse-derived sequences.