Ds. Zarlenga et al., ENZYMATIC AMPLIFICATION AND MOLECULAR-CLONING OF CDNA-ENCODING THE SMALL AND LARGE SUBUNITS OF BOVINE INTERLEUKIN-12, Biochimica et biophysica acta. Molecular basis of disease, 1270(2-3), 1995, pp. 215-217
cDNA generated from stimulated abomasal lymph node cells was used to a
mplify and clone the 35 kDa and 40 kDa subunits of bovine interleukin
12 (IL-12) using primers derived from semi-conserved regions between h
uman and mouse IL-12 sequences. The deduced amino acid sequence of the
40 kDa subunit demonstrated 84.4% and 67.6% homology with human and m
ouse sequences, respectively. The deduced sequence of the 35 kDa subun
it exhibited comparable similarities to the human 35 kDa subunit (82.2
%) but differed significantly (58.6%) from mouse-derived sequences.