E-CADHERIN IS INVOLVED IN THE INTRAHEPATIC METASTASIS OF HEPATOCELLULAR-CARCINOMA

In human hepatocellular carcinoma (HCC), the liver is the major target organ of metastasis, which is known as intrahepatic metastasis. To an alyze the mechanism of this metastasis, we established two sublines fr om the human HCG cell line Li7. Subline Li7HM produced multiple Liver metastasis, whereas subline Li7NM never did so after intrasplenic inje ction into nude mice. Two-dimensional gel electrophoresis and immunobl ot analysis showed that only Li7NM expressed vimentin and lacked E-cad herin expression, indicating that this clone had undergone epithelial- mesenchymal transition. We then transfected mouse E-cadherin complemen tary DNA into Li7NM cells and found that the transfectant cells (EM16. 21B.3) formed liver metastasis (8/16 mice) after intrasplenic injectio n and liver tumors (11/13 mice) after intrahepatic injection, whereas the control cell line formed no tumors, These results suggest that E-c adherin plays an important role in the process of intrahepatic metasta sis of HCC.