EFFECT OF CISPLATIN AND CIS-PLATINUM(II) PHOSPHONATE COMPLEX ON MURINE MAST-CELLS

We investigated the effect of two cis-platinum (II) complexes, cis-[Pt Cl2(NH3)(2)] (cisplatin) and cis-[PtCl2(4-pmpe)(2)] (4-pmpe stands for diethyl 4-pyridylmethylphosphonate), which was recently synthetized i n our laboratory, on murine mast cells. We noticed that both tested co mpounds were able to evoke histamine release from murine mast cells. T he histamine secretion was dependent on the concentration of compound and on the time and temperature of the reaction. It was also dependent on metabolic energy (the reaction was diminished in a medium without glucose and abolished in the presence of 2-deoxyglucose). The results indicate that cis-platinum (II) complexes activate mast cells to secre te histamine via a non-cytotoxic, active secretory process.