influxes of (NH4+)-N-13 across the root plasmalemma were measured in i
ntact seedlings of Picea glauca (Moench) Voss. Two kinetically distinc
t uptake systems for NH4+ were identified. In N-deprived plants, a Mic
haelis-Menten-type high-affinity transport system (HATS) operated in a
2.5 to 350 mu M range of external NH4+ concentration ([NH4+](o)). The
V-max of this HATS was 1.9 to 2.4 mu mol g(-1) h(-1), and the K-m was
20 to 40 mu M. At [NH4+](o) from 500 mu M to 50 mM, a linear low-affi
nity system (LATS) was apparent. Both HATS and LATS were constitutive.
A time-dependence study of NH4+ influx in previously N-deprived seedl
ings revealed a small transient increase of NH4+ influx after 24 h of
exposure to 100 mu M [NH4+](o). This was followed by a decline of infl
ux to a steady-state value after 4 d. In seedlings exposed to 100 mu M
external NO3- concentration for 3 d, the V-max for NH4+ uptake by HAT
S was increased approximately 30% compared to that found in N-deprived
seedlings, whereas LATS was down-regulated. The present study defines
the much higher uptake capacity for NH4+ than for NO3- in seedlings o
f this species.