F. Kontos et al., FACTORS AFFECTING ENDO-BETA-MANNANASE ACTIVITY IN THE ENDOSPERMS OF FENUGREEK AND CAROB SEEDS, Seed science research, 6(1), 1996, pp. 23-29
Endosperms of fenugreek and carob which have been leached following th
eir isolation exhibit increased activity of endo-beta-mannanase, part
of which is released into the surrounding incubation medium. This acti
vity was suppressed by the addition to the endosperms of abscisic acid
or endosperm/seed coat leachate. The leachate from carob inhibited th
e increase of endo-beta-mannanase activity in fenugreek and that from
fenugreek inhibited enzyme activity in carob but more weakly; neither
of these leachates inhibited the production of alpha-amylase in wheat
endosperm. Polyethylene glycol-induced stress on non-leached endosperm
s inhibited endo-beta-mannanase production, but not when the stress wa
s applied following endosperm leaching. However, the stress did reduce
the amount of enzyme released into the surrounding incubation medium.
Several isoenzymes of mannanase could be detected on isoelectric focu
sing activity gels in the endosperms of both fenugreek and carob, but
their pls differed between the two species. When activity was suppress
ed by ABA or leachate, all pi forms declined equally.