HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF PHENOL, 4-NITROPHENOL, BETA-NAPHTHOL AND A NUMBER OF THEIR GLUCURONIDE AND SULFATECONJUGATES IN ORGAN PERFUSATE

This paper describes a simple and more sensitive reversed-phase HPLC m ethod for the quantification of phenol, 4-nitrophenol and beta-naphtho l and some of their glucuronide and sulphate conjugates in aqueous sol ution and liver perfusate buffer. Methanol-water mobile phases with io n-pairing agents for each phenolic group are detailed. The assay showe d good recovery, accuracy and precision and is suitable for the quanti fication of these phenolic compounds in liver perfusion experiments.