QUANTITATION OF HOMOGENTISIC ACID IN NORMAL HUMAN PLASMA

Authors
DEUTSCH JC SANTHOSHKUMAR CR
Citation
Jc. Deutsch et Cr. Santhoshkumar, QUANTITATION OF HOMOGENTISIC ACID IN NORMAL HUMAN PLASMA, Journal of chromatography B. Biomedical applications, 677(1), 1996, pp. 147-151
Citations number
16
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
677
Issue
1
Year of publication
1996
Pages
147 - 151
Database
ISI
SICI code
Abstract
A new stable isotope dilution gas chromatograph-mass spectrometric met hod of analysis of homogentisic acid is described. Using this method, homogentisic acid is measured for the first time in normal human plasm a. The assay of sera from nine normal individuals yielded a range of v alues from 2.4 to 12 ng/ml. The method appears to be very sensitive an d may be useful in the characterization of heterozygotes for alkaptonu ria and other disorders of tyrosine degradation.