IN-SITU ANALYSIS OF NA,K-ATPASE ALPHA-1-ISOFORM AND ALPHA-3-ISOFORM MESSENGER-RNAS IN AGING RAT HIPPOCAMPUS

Citation
Nb. Chauhan et Gj. Siegel, IN-SITU ANALYSIS OF NA,K-ATPASE ALPHA-1-ISOFORM AND ALPHA-3-ISOFORM MESSENGER-RNAS IN AGING RAT HIPPOCAMPUS, Journal of neurochemistry, 66(4), 1996, pp. 1742-1751
Citations number
44
Categorie Soggetti
Biology,Neurosciences
Journal title
ISSN journal
00223042
Volume
66
Issue
4
Year of publication
1996
Pages
1742 - 1751
Database
ISI
SICI code
0022-3042(1996)66:4<1742:IAONAA>2.0.ZU;2-F
Abstract
Age-related changes in the expression of Na,K-ATPase alpha 1- and alph a 3-isoform mRNAs were analyzed by in situ hybridization in the Fische r-344 rat hippocampus. Quantification of signal density with cRNA prob es in rat hippocampus at 3 months of age showed (a) alpha 1 content is 1.5 times higher in granule than in pyramidal cell layers, whereas al pha 3 content shows the opposite ratio and (b) alpha 3 label is found in large clusters related to messy cells and basket cells and in mediu m clusters corresponding to interneurons within the dendritic fields o f CA1-3. In the 24-month-old rats as compared with the young animals, the alpha 1 signal is increased more than sevenfold in the dendritic f ields and is not significantly changed in perikaryal layers, The alpha 3 signal is reduced about threefold (p < 0.0001, ANOVA, n = 6) in per ikaryal layers, is almost completely absent over the interneurons, bas ket cells, and messy cells, and is not significantly changed in dendri tic fields, These data indicate age-related, cell- and isoform-specifi c alterations in pretranslational regulation of Na,K-ATPase alpha isof orms, The striking changes in the dendritic fields, messy cells, and G ABAergic basket cells and interneurons may constitute early and sensit ive markers for age-related alterations in hippocampal function, befor e cell loss.