PROTEIN BIOTINYLATION IN HIGHER-PLANTS - CHARACTERIZATION OF BIOTIN HOLOCARBOXYLASE SYNTHETASE-ACTIVITY FROM PEA (PISUM-SATIVUM) LEAVES

Authors
TISSOT G JOB D DOUCE R ALBAN C
Citation
G. Tissot et al., PROTEIN BIOTINYLATION IN HIGHER-PLANTS - CHARACTERIZATION OF BIOTIN HOLOCARBOXYLASE SYNTHETASE-ACTIVITY FROM PEA (PISUM-SATIVUM) LEAVES, Biochemical journal, 314, 1996, pp. 391-395
Citations number
38
Categorie Soggetti
Biology
Journal title
Biochemical journal → ACNP
ISSN journal
02646021
Volume
314
Year of publication
1996
Part
2
Pages
391 - 395
Database
ISI
SICI code
0264-6021(1996)314:<391:PBIH-C>2.0.ZU;2-3
Abstract
Biotin holocarboxylase synthetase was partially purified from pea leav es by a sequence of ammonium sulphate fractionation and DEAE 52-cellul ose chromatography. Enzyme activity was assayed using apo-(biotin carb oxyl carrier protein) from an Escherichia coli bir A mutant affected i n biotin holocarboxylase synthetase activity. Conditions for optimal c atalytic activity and biochemical parameters of the plant enzyme were determined. This is the first direct evidence of the existence of biot in holocarboxylase synthetase activity in plants.