Jm. Vanrooij et Jj. Videler, A SIMPLE FIELD METHOD FOR STEREO-PHOTOGRAPHIC LENGTH MEASUREMENT OF FREE-SWIMMING FISH - MERITS AND CONSTRAINTS, Journal of experimental marine biology and ecology, 195(2), 1996, pp. 237-249
A simple field method to correct for bias in stereo-photographic under
water measurements is presented and its usefulness to improve the accu
racy of length estimates of free-swimming fish is tested. The calibrat
ion is based on the inclusion of stereo exposures of a slate of known
dimensions at the beginning and end of each measuring session. and it
allows correction for non-parallel optical axes, spherical aberration
of the lenses, and for other sources of bias that may vary between ses
sions. A precision of +/-3% for replicate length measurements of a 30
cm slate is obtained at distances between 0.7 and 2.0 m. This compares
well with reported values that are obtained with more sophisticated (
laboratory) methods. However, application of this calibration does not
significantly improve the accuracy of stereo measurements of the leng
th of free-swimming fish (+/-2.5 cm or 7-11% of actual fish length), T
he limitations are caused by difficulties in recognizing identical ext
reme points at the body of fish that are photographed under field cond
itions. By averaging three or more replicate measurements the accuracy
is improved to +/-1 cm (2.7-4.5%). We conclude that effort should be
aimed at increasing sample size, rather than at improving equipment an
d correction procedures, when measuring free-swimming fish in their na
tural habitat.