GUIDANCE AND ACTIVATION OF MURINE MACROPHAGES BY NANOMETRIC SCALE TOPOGRAPHY

Citation
B. Wojciakstothard et al., GUIDANCE AND ACTIVATION OF MURINE MACROPHAGES BY NANOMETRIC SCALE TOPOGRAPHY, Experimental cell research, 223(2), 1996, pp. 426-435
Citations number
25
Categorie Soggetti
Oncology,"Cell Biology
Journal title
ISSN journal
00144827
Volume
223
Issue
2
Year of publication
1996
Pages
426 - 435
Database
ISI
SICI code
0014-4827(1996)223:2<426:GAAOMM>2.0.ZU;2-F
Abstract
We studied the guidance and activation of macrophages from the P388D1 cell line and rat peritoneum by topographic features on a nanometric s cale, Cells were plated on plain fused silica substrata or substrata w ith microfabricated grooves and steps, 30-282 nm deep, The contact of cells with the patterned surface activated cell spreading and adhesion and increased the number of protrusions of the cell membrane. These c hanges were accompanied by an increase in the amount of F-actin in cel ls, The accumulation of F-actin and vinculin in cells was observed alo ng the edges of single steps or grooves, Formation of focal contacts a long discontinuities in the substratum was accompanied by the phosphor ylation of tyrosine colocalized with F-actin and vinculin, A similar p attern of staining was seen in cells stained for vitronectin receptor, alpha V integrin, but not for integrins alpha 5 beta 1 or alpha 3 bet a 1, Cells cultured on nanogrooves showed a higher phagocytotic activi ty than cells cultured on plain substrata, We show that macrophages ca n react to ultrafine features of topography of a size comparable to th at of a single collagen fiber and become activated by the contact with topographic features. (C) 1996 Academic Press, Inc.