THE PASTEURELLA-HAEMOLYTICA O-SIALOGLYCOPROTEIN ENDOPEPTIDASE IS INHIBITED BY ZINC IONS AND DOES NOT CLEAVE FETUIN

Authors
CLADMAN WM WATT MAV DINI JP MELLORS A
Citation
Wm. Cladman et al., THE PASTEURELLA-HAEMOLYTICA O-SIALOGLYCOPROTEIN ENDOPEPTIDASE IS INHIBITED BY ZINC IONS AND DOES NOT CLEAVE FETUIN, Biochemical and biophysical research communications, 220(1), 1996, pp. 141-146
Citations number
15
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
220
Issue
1
Year of publication
1996
Pages
141 - 146
Database
ISI
SICI code
0006-291X(1996)220:1<141:TPOEII>2.0.ZU;2-T
Abstract
Culture supernatants of Pasteurella haemolytica A1 contain an O-sialog lycoprotein endopeptidase that cleaves human glycophorin A. This enzym e is inhibited by micromolar concentrations of Zn2+. It can be separat ed from a neuraminidase activity in culture supernatants by ion-exchan ge chromatography. The neuraminidase activity can cause the de-sialati on of the bovine soluble sialoglycoprotein, fetuin. However fetuin is not cleaved proteolytically either by culture supernatants from P. hae molytica A1 or by chromatographically purified O-sialoglycoprotein end opeptidase or neuraminidase. (C) 1996 Academic Press, Inc.