K. Yamazoe et al., SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OFCARBOPLATIN, EPIRUBICIN HYDROCHLORIDE AND MITOMYCIN-C IN A LIPIODOL EMULSION, Journal of chromatography, 726(1-2), 1996, pp. 241-245
Citations number
17
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A novel HPLC method for the simultaneous determination of carboplatin
(CBDCA), epirubicin hydrochloride (EPI) and mitomycin C (MMC) was deve
loped in order to determine these drugs in a Lipiodol emulsion for int
ra-arterial injection and in the medium of a drug-release test of the
emulsion. The HPLC conditions included a reversed-phase column and a g
radient programme of the mobile phase using 0.01 M phosphate buffer (p
H 3.0)-acetonitrile. The accuracies of analysis for each drug added to
the emulsion and the drug-release medium were in the ranges 96-104% a
nd 98-108%, respectively. The R.S.D.s in the repeatability of the dete
rmination of each drug in the emulsion and in the drug-release medium
were <8% and <2%, respectively. The method is simple and reliable enou
gh to be utilized for the investigation of drug contents and drug rele
ase of the Lipiodol emulsion containing CBDCA, EPI and MMC.