SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OFCARBOPLATIN, EPIRUBICIN HYDROCHLORIDE AND MITOMYCIN-C IN A LIPIODOL EMULSION

A novel HPLC method for the simultaneous determination of carboplatin (CBDCA), epirubicin hydrochloride (EPI) and mitomycin C (MMC) was deve loped in order to determine these drugs in a Lipiodol emulsion for int ra-arterial injection and in the medium of a drug-release test of the emulsion. The HPLC conditions included a reversed-phase column and a g radient programme of the mobile phase using 0.01 M phosphate buffer (p H 3.0)-acetonitrile. The accuracies of analysis for each drug added to the emulsion and the drug-release medium were in the ranges 96-104% a nd 98-108%, respectively. The R.S.D.s in the repeatability of the dete rmination of each drug in the emulsion and in the drug-release medium were <8% and <2%, respectively. The method is simple and reliable enou gh to be utilized for the investigation of drug contents and drug rele ase of the Lipiodol emulsion containing CBDCA, EPI and MMC.