SENSITIVE PCR DIAGNOSIS OF INFECTIONS BY ENTEROCYTOZOON-BIENEUSI (MICROSPORIDIA) USING PRIMERS BASED ON THE REGION CODING FOR SMALL-SUBUNITRIBOSOMAL-RNA

Citation
Aj. Dasilva et al., SENSITIVE PCR DIAGNOSIS OF INFECTIONS BY ENTEROCYTOZOON-BIENEUSI (MICROSPORIDIA) USING PRIMERS BASED ON THE REGION CODING FOR SMALL-SUBUNITRIBOSOMAL-RNA, Journal of clinical microbiology, 34(4), 1996, pp. 986-987
Citations number
10
Categorie Soggetti
Microbiology
ISSN journal
00951137
Volume
34
Issue
4
Year of publication
1996
Pages
986 - 987
Database
ISI
SICI code
0095-1137(1996)34:4<986:SPDOIB>2.0.ZU;2-4
Abstract
Enterocytozoon bieneusi is the most common microsporidian infecting pa tients with AIDS. We have developed a PCR primer pair, named EBIEF1/EB IER1, based on the small-subunit rRNA sequence of this microsporidian. Compared with other PCR-based methods, this primer pair shows a highe r efficiency of detection in diagnostic applications than does another previously described primer pair, V1/EB450.