T. Mizote et al., GEL MOBILITY SHIFT SCANNING OF THE ACETATE INDUCIBLE PROMOTERS FROM NEUROSPORA-CRASSA REVEALS A COMMON CO INDUCIBLE DNA-BINDING PROTEIN, MGG. Molecular & general genetics, 250(4), 1996, pp. 421-427
The promoter regions of four acetate-inducible genes of Neurospora cra
ssa, acu-3, acu-5, acu-8 and acu-9, have been sequenced. Using a scann
ing gel mobility shift assay particular DNA regions in each promoter h
ave been shown specifically to bind partially purified protein extract
ed from acetate-induced mycelia. The protein-binding regions so define
d have common sequence motifs, elements of which are similar to those
required for acetate induction in Aspergillus nidulans.