GEL MOBILITY SHIFT SCANNING OF THE ACETATE INDUCIBLE PROMOTERS FROM NEUROSPORA-CRASSA REVEALS A COMMON CO INDUCIBLE DNA-BINDING PROTEIN

The promoter regions of four acetate-inducible genes of Neurospora cra ssa, acu-3, acu-5, acu-8 and acu-9, have been sequenced. Using a scann ing gel mobility shift assay particular DNA regions in each promoter h ave been shown specifically to bind partially purified protein extract ed from acetate-induced mycelia. The protein-binding regions so define d have common sequence motifs, elements of which are similar to those required for acetate induction in Aspergillus nidulans.