DEVELOPMENT OF A STAND-ALONE AFFINITY CLEANUP FOR LYSERGIC-ACID DIETHYLAMIDE IN URINE

A total analysis scheme for lysergic acid diethylamide (LSD) from huma n urine is described. A simple ELISA technique led to the development and optimization of an affinity clean-up cartridge, resulting in high purification factors with a single combined extraction/clean-up step. LSD can be measured with a straightforward HPI,C-fluorescence techniqu e, which minimizes operating complexity and process implementation tim e. The method has been applied to urine containing 0.5 ng ml(-1) LSD, and the ability of high-affinity materials to preconcentrate a sample into a small volume should allow the working range of the procedure to be adjusted as required.