A total analysis scheme for lysergic acid diethylamide (LSD) from huma
n urine is described. A simple ELISA technique led to the development
and optimization of an affinity clean-up cartridge, resulting in high
purification factors with a single combined extraction/clean-up step.
LSD can be measured with a straightforward HPI,C-fluorescence techniqu
e, which minimizes operating complexity and process implementation tim
e. The method has been applied to urine containing 0.5 ng ml(-1) LSD,
and the ability of high-affinity materials to preconcentrate a sample
into a small volume should allow the working range of the procedure to
be adjusted as required.