In this study we followed the behavior of germ cell precursors in the
early embryo of the dipteran Ceratitis capitata using conventional flu
orescence, laser scanning confocal and transmissiom electron microscop
ies, During cellularization the pole cells formed a cluster which lodg
ed in a roundish break in the blastoderm at the posterior pole of the
embryo, When gastrulation began, the pole cells shifted dorsally and d
uring elongation of the germ band moved into the posterior midgut prim
ordium. Pole cell morphology suggested that these cells were motile un
til the early stages of development.