ENANTIOMERIC SEPARATION OF 6 2-ARYLPROPIONIC ACIDS AFTER PRECOLUMN DERIVATIZATION WITH VARIOUS AMINES AND ALCOHOLS ON A CELLULOSE-BASED CHIRAL STATIONARY-PHASE

A large number of mono-, bi- and tricyclic amines and alcohols were us ed for the derivatization of six 2-arylpropionic acids. The effect of the bonded aromatic portion on the enantiomeric separation using a tri s(4-methylbenzoate)cellulose column was studied. Small modifications o f the bonded groups close to the chiral centre pointed out their predo minant steric effect to the chiral recognition process rather than ele ctronic influence. Varying ratios of methanol and perchlorate buffer c omposing the mobile phase were applied to elute the different series w ithin comparable analysis times.