BACTERIA-BASED BIOSENSOR FOR DETERMINATION OF HYDROGEN-PEROXIDE

A bacterial biosensor that immobilizes living bacteria of strain Bacil lus subtilis AS 1.398 are coupled to a Clark-type oxygen electrode to determine hydrogen peroxide. The H2O2 is catalyzed by catalase from th e bacteria to produce H2O and O-2. This sensor is advantageous in term s of linearity (10 to 2000 mu M H2O2), lifetime (65 days), and selecti vity (no significant interference from some amino acids, metal ions, p olyphenols, glucose, and ascorbic acid), especially because bacteria c an be used as 2 catalase enzyme membrane that can reproduce itself. Th e correlation coefficient is 0.999. (C) 1996 Academic Press, Inc.