EXPRESSION OF THE ARXULA-ADENINIVORANS GLUCOAMYLASE GENE IN KLUYVEROMYCES-LACTIS

The glucoamylase gene of the yeast Arxula adeninivorans was expressed in Kluyveromyces lactis by using the GAP promoter from Saccharomyces c erevisiae and a multicopy plasmid vector. The transformants secreted 9 0.1% of the synthesized glucoamylase into the culture medium. The secr eted glucoamylase activities are about 20 times higher in comparison t o those of Saccharomyces cerevisiae transformants using the same promo ter. Secreted glucoamylase possesses identical N-terminal amino acid s equences to those secreted by A. adeninivorans showing that cleavage o f the N-terminal signal peptide takes place at the same site. Biochemi cal characteristics of glucoamylase expressed by K. lactis and A. aden inivorans are very similar.