A. Kunjumon et al., PLANT-REGENERATION FROM LEAF EXPLANTS OF 3 GRAIN LEGUMES ON SAME MEDIUM, Journal of Plant Biochemistry and Biotechnology, 5(1), 1996, pp. 27-29
The regeneration potential of leaves of moth bean, pigeonpea and grass
pea was studied on Murashige and Skoog's (MS) medium supplemented wit
h various combinations of auxins and cytokinins. Regeneration of shoot
s was obtained mainly on MS medium supplemented with high auxin and lo
w cytokinin levels. However, frequency of response varied not only in
the three legume species but also in their varieties studied. Roots we
re induced on regenerated shoots by transferring them to MS basal medi
um. Complete plant regeneration was observed in the three legumes in a
short duration of 60-70 days.