S. Mohandas et Ms. Shaila, DETECTION OF NIF GENES IN NITROGEN-FIXING BACTERIAL ISOLATE FROM TOMATO (LYCOPERSICON-ESCULENTUM MILL CV PUSA RUBY), Journal of Plant Biochemistry and Biotechnology, 5(1), 1996, pp. 59-60
Nitrogen-fixing bacterial isolate from the intercellular spaces of tom
ato root cortical cells was studied for the location of nif genes on t
he chromosomal or plasmid DNA. The bacterial isolate showed two plasmi
ds of approximate molecular sizes of 220 and 120 kb. Klebsiella pneumo
niae nif HDK probe hybridized with the chromosomal DNA and not with th
e plasmid DNA thereby showing that nif genes are localised on the chro
mosomal DNA.