INTERACTION OF GTP WITH PROTEINS DURING THE CELL-CYCLE OF STREPTOMYCES-COELICOLOR

Six putative GTP binding proteins were detected by ultraviolet light i n the presence of [alpha-P-32]GTP during the developmental cycle of St reptomyces coelicolor. Four out of six were true GTP binding proteins. Immunological reactions carried out with antiserum which recognizes t he alpha-common subunit of G regulatory proteins identified two bands of 67 kDa and 30 kDa. Studies with [gamma-P-32]GTP showed significant changes in protein phosphorylation during the cell cycle. The results show that at least three different systems of GTP protein interaction are present in S. coelicolor.