Mm. Yousfi et al., DETECTION OF HELICOBACTER-PYLORI BY RAPID UREASE TESTS - IS BIOPSY SIZE A CRITICAL VARIABLE, Gastrointestinal endoscopy, 43(3), 1996, pp. 222-224
Background: The variables responsible for false-positive and false-neg
ative rapid urease tests are largely unexplored. Objectives: We compar
ed the results of rapid urease testing with jumbo cup forceps (3.3 mm
diameter) and tiny cup forceps (1.8 mm diameter) with two rapid urease
tests. Methods: Antral biopsies were obtained. The order of forceps a
nd rapid urease tests was randomized. Biopsies were also taken for Gen
ta staining. Results: One hundred and two patients were studied; 59 ha
d Helicobacter pylori infection. There were 22 false-negative tests (8
CLOtest, 16 hpfast) and 5 false-positive tests (3 CLOtest, 2 hpfast).
All 5 false-positive tests were among those positive only in the seco
nd 12 hours. There was no difference in results with the jumbo and tin
y cup forceps. Five percent to nine percent (average, 6.6%; 95% confid
ence interval, 4.4% to 9.6%) of tests with any of the combinations gav
e an erroneous categorization of H. pylori status. There were no clini
cal or statistical differences in H. pylori categorizations by CLOtest
or hpfast. Conclusion: The diagnostic yield for detecting H. pylori i
nfection by rapid urease tests is not adversely affected by small biop
sy size, possibly because tiny biopsies obtain superficial tissue wher
e H. pylori reside.