EVALUATION OF NONISOTOPIC IN-SITU HYBRIDIZATION FOR MESSENGER-RNA IN REACTIVE AND NEOPLASTIC LYMPHOID-CELLS

Authors
PITERA R PITERA JE SALISBURY JR
Citation
R. Pitera et al., EVALUATION OF NONISOTOPIC IN-SITU HYBRIDIZATION FOR MESSENGER-RNA IN REACTIVE AND NEOPLASTIC LYMPHOID-CELLS, Pathology research and practice, 192(2), 1996, pp. 130-136
Citations number
16
Categorie Soggetti
Pathology
Journal title
Pathology research and practice → ACNP
ISSN journal
03440338
Volume
192
Issue
2
Year of publication
1996
Pages
130 - 136
Database
ISI
SICI code
0344-0338(1996)192:2<130:EONIHF>2.0.ZU;2-X
Abstract
An evaluation of nonisotopic in situ hybridization (NISH) for mRNA in archival lymphoid tissue was carried out and an analysis of factors af fecting the final outcome was performed. A modification of the in situ reverse transcription method for RNA preservation assessment has been used and described. We have sh own that, for frozen samples mRNA dete ction is optimal within 3 months of the biopsy being taken, while pres ervation declines after 1 year of storage.