A microcomputer-based video system for tracking, recording, and analyz
ing the movement of animals in two dimensions on variegated background
in real-time has been developed and validated, both mechanically and
with moving animals. Hardware and software (donationware) costs are lo
w, Specimens visualized as small as 3 pixels long may be tracked in an
arena that is 240 x 320 pixels in size. The results of tracking a mec
hanical moving spot are compared with a theoretical circular path. Com
parisons between frame-by-frame human observation and computer generat
ed X-Y coordinates are also presented. Applications of the tracking sy
stem include insect toxicology and pheromone bioassay, vertebrate loco
motion studies, and basic research on taxes and kineses.