The activity of triazole fungicides towards Botrytis cinerea was inves
tigated in vitro (radial growth on fungicide-amended agar) and in vivo
(foliar-sprayed tomato plants and dip-treated grapes). In both tests
the benzimidazoles, benomyl and thiabendazole, and the dicarboximides,
iprodione and vinclozolin, were used as reference fungicides. In all
experiments benomyl and tebuconazole proved to be the most active fung
icides. The transfer ratio, which is defined as the ratio between the
EC(50) (the concentration inhibiting growth by 50%) of a particular fu
ngicide determined in vivo and in vitro, was lowest for benomyl. The t
ransfer ratio of tebuconazole was comparable to or lower than that of
vinclozolin. Hence, no obvious correlation between in vitro and in viv
o activity was observed. Field rates of tebuconazole recommended for c
ontrol of B. cinerea are relatively low compared to those of benzimida
zoles and dicarboximides. Tomato leaf homogenates and various biologic
al compounds antagonized the activity of triazoles and dicarboximides
but did not affect inhibitory potency of benzimidazoles. It is suggest
ed that the factors mentioned account only partly for the limited fiel
d performance of triazoles towards B. cinerea.