SHOOT REGENERATION IN LONG-TERM CALLUS-CULTURES DERIVED FROM MATURE FLOWERING PLANTS OF CYCLAMEN-PERSICUM MILL

In long-term callus cultures of Cyclamen persicum Mill, two types of t issue could be distinguished. One type featured a brown suberised oute r layer and was poorly organogenic, The other type was yellowish in ap pearance and gave rise to many shoot buds, Both types co-existed on th e same callus, the former prevailing. Selection for organogenic tissue resulted in cultures yielding approximately three times more petioles than random subcultures. Callus-derived shoots could be rooted and es tablished in the greenhouse. The method allowed for the production of thousands of plants but the regenerants often showed deviant phenotype s and genotypes.