W. Dillen et al., SHOOT REGENERATION IN LONG-TERM CALLUS-CULTURES DERIVED FROM MATURE FLOWERING PLANTS OF CYCLAMEN-PERSICUM MILL, Plant cell reports, 15(7), 1996, pp. 545-548
In long-term callus cultures of Cyclamen persicum Mill, two types of t
issue could be distinguished. One type featured a brown suberised oute
r layer and was poorly organogenic, The other type was yellowish in ap
pearance and gave rise to many shoot buds, Both types co-existed on th
e same callus, the former prevailing. Selection for organogenic tissue
resulted in cultures yielding approximately three times more petioles
than random subcultures. Callus-derived shoots could be rooted and es
tablished in the greenhouse. The method allowed for the production of
thousands of plants but the regenerants often showed deviant phenotype
s and genotypes.