IN-VITRO REPAIR OF OXIDATIVE AND ULTRAVIOLET-INDUCED DNA-DAMAGE IN SUPERCOILED NUCLEOID DNA BY HUMAN CELL EXTRACT

Authors
COLLINS AR FLEMING IM GEDIK CM
Citation
Ar. Collins et al., IN-VITRO REPAIR OF OXIDATIVE AND ULTRAVIOLET-INDUCED DNA-DAMAGE IN SUPERCOILED NUCLEOID DNA BY HUMAN CELL EXTRACT, Biochimica et biophysica acta, N. Gene structure and expression, 1219(3), 1994, pp. 724-727
Citations number
11
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1219
Issue
3
Year of publication
1994
Pages
724 - 727
Database
ISI
SICI code
0167-4781(1994)1219:3<724:IROOAU>2.0.ZU;2-H
Abstract
A human cell extract is capable of carrying out damage-specific incisi on on supercoiled nucleoid DNA. The DNA breaks are detected by single cell gel electrophoresis. Incubation of the incised DNA with extract a nd DNA precursors results in resealing of the breaks, with retention o f the supercoiled state of the DNA.