Peroxidases of different origin - horseradish peroxidase isozyme C, al
falfa and peanut cationic peroxidases, tobacco leaves and novel fungal
anionic peroxidases - were used to determine phenol and its analogues
. Phenol and resorcinol were shown to be the inhibitors of the peroxid
ase activity towards o-dianisidine for all the enzymes tested, whereas
pyrogallol and hydroquinone caused an appearance of a lag-period on a
kinetic curve. The duration of a lag-period was proportional to the e
ffector concentration and could be used to determine it. The novel fun
gal peroxidase from Phellinus igniarius exhibited the highest sensitiv
ity towards phenols and they could be determined at the 10-6 - 10-7 M
concentration levels.