BIOLUMINESCENT ANALYSIS - THE ACTION OF TOXICANTS - PHYSICAL-CHEMICALREGULARITIES OF THE TOXICANTS EFFECTS

The physical-chemical regularities of aromatic compounds' effects in l uciferase to toxicity biotesting have been studied, The structures and physical-chemical characteristics of the toxicants and of the biolumi nescent emitter were taken into account. The inhibition constants of b ioluminescence intensity (I) were calculated and interpreted from the viewpoint of the energy (electron) transfer processes. The induction p eriod (P) and the increase of the rime of the maximum light intensity (t(M)) which take place in the quinones presence, have been shown to d eal with hydrogen transfer processes. The values of I, P and t(M) have been shown to be connected with a size of the quinones' aromatic and aliphatic parts, P- and t(M)-dependencies on quinone's redox potential have been demonstrated.