LACCASE AS A NEW ENZYMATIC LABEL FOR ENZYME-IMMUNOASSAY

A new immunochemical reagent is suggested containing as an enzyme mark er laccase obtained from the cultural liquid of basidial fungi Coriolu s hirsutus. The feasibility of immuno-Iaccase conjugates in different versions of immunoanalysis (sandwich, competitive and indirect enzyme immunoassay) was demonstrated. The assay based on antibodv-laccase con jugates is simpler than that employing antibody-peroxidase conjugates, since in the former case air oxygen is used as the second substrate o f the enzymatic reaction.