COMPLETE SEQUENCE OF THE GENE ENCODING THE BACTERIOFERRITIN SUBUNIT OF MYCOBACTERIUM-AVIUM SUBSPECIES SILVATICUM

Authors
INGLIS NF STEVENSON K HOSIE AHF SHARP JM
Citation
Nf. Inglis et al., COMPLETE SEQUENCE OF THE GENE ENCODING THE BACTERIOFERRITIN SUBUNIT OF MYCOBACTERIUM-AVIUM SUBSPECIES SILVATICUM, Gene, 150(1), 1994, pp. 205-206
Citations number
9
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
150
Issue
1
Year of publication
1994
Pages
205 - 206
Database
ISI
SICI code
0378-1119(1994)150:1<205:CSOTGE>2.0.ZU;2-4
Abstract
A gene encoding the bacterioferritin subunit (Bfr) of Mycobacterium av ium (Ma) subspecies silvaticum has been cloned, sequenced and expresse d. The 477-bp open reading frame codes for 159 amino acids, which were shown to share up to 92% identity with the Bfr of five bacterial gene ra. The recombinant Bfr exhibits serological cross-reactivity with Ma paratuberculosis antigen D, a protein of approx. 20 kDa in cell lysate s of Ma paratuberculosis and Ma silvaticum and a protein of 20-22 kDa in sonicates of M. leprae.