GASTRIN STIMULATES HISTAMINE-RELEASE FROM THE ISOLATED PIG STOMACH

Background: Gastrin has been shown to give an immediate and dose-depen dent histamine release preceding acid secretion in the totally isolate d, vascularly perfused rat stomach. Methods: In the present study we p repared isolated, vascularly perfused stomachs taken from pigs and exa mined the effect of pentagastrin in a concentration of 520 pM on the h istamine release to the vascular bed. Pentagastrin was given for three 5-min periods at 10-min intervals, and histamine was determined by a radioimmunoassay method. Results: Each pentagastrin dose resulted in s timulation of histamine release in the three pig stomachs examined. Th e various histamine concentrations increased to levels previously show n to stimulate aminopyrine accumulation in isolated pig parietal cells . Conclusion: The present study shows for the first time that gastrin induces histamine release to the vascular bed in the pig, as previousl y shown for the rat and dog.