A. Guzaev et al., SYNTHESIS AND PRIMER PROPERTIES OF OLIGONUCLEOTIDES CONTAINING 3'-DEOXYPSICOTHYMIDINE UNITS, LABELED WITH FLUORESCEIN AT THE 1'-POSITION, Bioconjugate chemistry, 5(6), 1994, pp. 501-503
Several analogues of the standard M13 sequencing primer that contain u
p to five 3'-deoxypsicothymidines, or one or two such units labeled wi
th fluorescein at the 1'-position, have been prepared. All these oligo
nucleotides have been shown to prime the DNA-polymerase-catalyzed synt
hesis of DNA.