REACTION OF 4-HYDROXYNONENAL WITH SOME THIOL-CONTAINING RADIOPROTECTIVE AGENTS OR THEIR ACTIVE METABOLITES

The rate of reaction of several radioprotective agents or their active metabolites with 4-hydroxynonenal (4HNE) was studied and compared to the rate of reaction with cysteine (Cys) and glutathione (GSH). The ag ents studied were: mercapto ethylamine (MEA); 2(3-aminopropyl) aminoet hanethiol (WR1065); S-2-aminoethylisothiouronium bromide-hydrobromide (AET); 1,4-dithiothreitol (DTT); 1,4-dithioerythritol (DTE); N 2(2-mer captopropionyl) -glycine (MPG); penicillamine hydro chloride (PA); N-a cetylcysteine (NAC); 2-3 dimercapto-1 propane sulfonic acid (DMPS); 2, 3-dimercaptopropanol (BAL), and meso 2,3 dimercapto succinic acid (DMS ). All of them reacted with 4HNE. MEA and WR1065 were the most reactiv e thiols, and PA and DMS were the least reactive thiols. All the other s reacted at rates comparable to or higher than that of cysteine or GS H. The potential role of this type of interactions in the protective a ction of these drugs against deleterious effects of radiation or carbo n tetrachloride is analyzed.