APPLICATION OF A TRANSFORMED-CELL LINE CONSTITUTIVELY EXPRESSING HSV-1 POLYPEPTIDES FOR THE DETECTION OF HSV ANTIBODIES IN HUMAN SERA BY ANENZYME-IMMUNOASSAY
A. Sivropoulou et al., APPLICATION OF A TRANSFORMED-CELL LINE CONSTITUTIVELY EXPRESSING HSV-1 POLYPEPTIDES FOR THE DETECTION OF HSV ANTIBODIES IN HUMAN SERA BY ANENZYME-IMMUNOASSAY, Archives of virology, 139(1-2), 1994, pp. 183-188
We have previously reported the construction of a cell line BA4, const
itutively producing the glycoproteins go, gG, and alpha 4, the major r
egulatory protein of HSV-1. These cells have been selected in stepwise
increasing concentrations of methotrexate and shown to produce much h
igher amounts of go than non-selected cells. Extracts of the selected
cells were used in an enzyme linked immunosorbent assay to detect HSV
antibodies in human sera obtained from Greek blood donors. We report h
ere that (i) the assay developed is able to distinguish HSV antibody p
ositive from negative human sera and (ii) that its application in an e
pidemiological survey showed that the incidence of HSV infection in th
e general population in Greece is 90.4%.