GENERALIZED CONSENSUS MATRIX FOR RECOGNITION OF CLEAVAGE POLYADENYLATION SITES IN VERTEBRATE PRE-MESSENGER-RNA/

A sample of 63 sequences of the cleavage/polyadenylation sites in vert ebrate pre-mRNA was used to construct a generalized consensus matrix, composed of the absolute frequencies of oligonucleotides of length l i n the ith position relative to the cleavage point. To recognize a poly adenylation site in a nucleotide sequence, a multiplicative measure wa s obtained using the matrical elements as weighing factors. For any om ega-long segment, comparison of the functional mu with the threshold v alue mu permits one to judge whether the given segment is a processin g site.