The role of fungi, true bacteria and streptomycetes in the decay of ne
w and historical parchment is discussed. True bacteria usually attack
strongly humudified or inundated parchement turning it into a darkened
sticky mass. On parchment stored under high relative air humidity con
ditions (95 - 100%) there appear numerous colonies of fungi (Aspergill
us sp., Epicoccum sp., Penicillium sp. etc.) and streptomycetes causin
g coloured stains and holes. The collagen losses caused by various gro
ups of microorganisms were determined. SEM investigations of deteriora
ted parchment samples revealed deep damages of collagen fibres (pits,
cracks, splittings). The active role of streptomycetes in the decompos
ing of parchment is underlined.