FACTORS AFFECTING INTRA AND EXTRACELLULAR PHOSPHOLIPASE-A PRODUCTION BY SALMONELLA-TYPHI

The effect of various physico-chemical factors on the production of in tra and extracellular phospholipase A by Salmonella typhi was investig ated. Maximum intracellular enzyme levels were observed when cells wer e grown in brain heart infusion broth, after 16h of incubation at 37-d egrees-C. Highest level of extracellular phospholipase A was also seen in the same medium (pH 7.0), but after 24 h of incubation at 37-degre es-C. Agitation during incubation enhanced the enzyme systhesis. Addit ion of surfactants to the growth media significantly decreased both in tra and extracellular phospholipase A production.