REACTION-MECHANISM OF ISOMALTOOLIGOSACCHARIDES SYNTHESIS BY ALPHA-GLUCOSIDASE FROM ASPERGILLUS-CARBONARIOUS

An alpha-glucosidase from Aspergillus carbonarious CCRC 30414 was empl oyed for investigating the enzymatic synthesis of isomalntooligosaccha rides from maltose. The enzyme transferred a glucose unit from the non reducing end of maltose and other alpha-linked glucosyl oligosaccharid es to glucose and other glucosyl oligosaccharides which function as ac cepting co-substrates. The transfer of a glucose unit occurs most freq uently to the 6-OH position of the nonreducing end of acceptor, but tr ansfer to 4-OH position also occurs. Treatment of 30 % (w/v) maltose w ith the enzyme under optimum conditions afforded more than 50 % isomal tooligosaccharides.