P. Cazabonne et al., FAILURE OF MOUSE PERITONEAL MACROPHAGE ACTIVATION BY THE PURIFIED EXCRETED SECRETED ANTIGENS OF TOXOPLASMA-GONDII/, Microbiology and immunology, 38(11), 1994, pp. 909-913
This study was carried out to evaluate the effect of excreted/secreted
antigens on macrophages infected by Toxoplasma gondii. Six proteins 2
8, 30, 45, 58, 63 and 145 kDa were separated by different chromatograp
hic techniques. Mouse peritoneal macrophages were treated in vitro and
in vivo with these purified fractions. Penetration and proliferation
assays of T. gondii in the macrophages were performed in vitro. The di
fferent antigens used did not change the rate of penetration and proli
feration of the parasites. Therefore, the secreted products, which are
capable of provoking an immune response, could not directly activate
the macrophages. Furthermore, the secreted products were not cytotoxic
and neither did they possess a visible phospholipasic activity which
would have increased penetration.