Nc. Smith et al., MATERNAL TRANSFER OF ANTIBODIES INDUCED BY INFECTION WITH EIMERIA-MAXIMA PARTIALLY PROTECTS CHICKENS AGAINST CHALLENGE WITH EIMERIA-TENELLA, Parasitology, 109, 1994, pp. 551-557
Infection of breeding hens with Eimeria maxima induces production of E
imeria-specific IgG antibodies which are transferred to hatchlings via
the egg yolk and confer a high degree of maternal immunity against ho
mologous challenge and partial immunity to infection with another impo
rtant species, Eimeria tenella. As an example, in an experiment using
hatchlings from eggs collected between days 28 and 39 after infection
of the hens with 20 000 sporulated E. maxima oocysts, control chicks (
challenged with 100 sporulated oocysts) excreted 6.8+/-1.2 million (me
an +/-S.E., n = 10) or 5.8+/-1.2 million (n = 8) oocysts of E. maxima
or E. tenella, respectively, compared to 0.9+/-0.4 million (n = 5) E.
marima oocysts or 2.2+/-0.4 million (n = 9) E. tenella oocysts excrete
d by hatchlings of infected hens. This represents an 87% reduction in
oocyst excretion with regard to E. maxima and a 62% reduction in oocys
t excretion with regard to E. tenella in the progeny of the infected h
ens. In another experiment, eggs were collected from days 28 to 37 and
again from days 114 to 123 after infection of the hens with E. maxima
and hatchling oocyst excretion rates were 82% and 62%, respectively,
reduced for E. maxima and 43% and 41%, respectively, reduced for E. te
nella in the progeny of hens infected with E. maxima compared to the p
rogeny of uninfected hens. ELISA and Western blot analyses of maternal
ly-derived IgG revealed a high degree of cross-reactivity to antigens
of E. maxima and E. tenella. Thus, maternally-derived, IgG-mediated cr
oss-resistance to different species of Eimeria occurs in the chicken,
most likely as a result of cross-recognition of conserved epitopes or
proteins.