THE BDNF CONTENT OF POSTNATAL AND ADULT-RAT BRAIN - THE EFFECTS OF 6-HYDROXYDOPAMINE LESIONS IN ADULT BRAIN

Brain-derived neurotrophic factor (BDNF) has been well studied for its effects in improving survival and differentiation of the central and peripheral nervous systems. In order to understand the developing CNS and the pathogenesis of brain injury, an enzyme immunoassay was employ ed to detect BDNF protein in the various tissues of developing and adu lt animals. Increased levels of BDNF were found in the hippocampus, ce rebrocortex, striatum, cerebellum and ventral mesencephalon in 2-week- old rats, compared with that in postnatal day 0 pups. In the adult rat , the highest level of BDNF was detected in the hippocampus (14.5 +/- 0.8 ng/g wet tissue), with a relatively high level also observed in th e cerebrocortex and striatum. In peripheral tissues, a substantial amo unt of BDNF protein was observed in various organs. The changes in BDN F levels in the striatum and the ventral mesencephalon of unilaterally 6-hydroxydopamine-lesioned young adult rats were also examined. Signi ficant increases of BDNF levels were detected during 2 weeks after les ion.